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超低频经颅磁刺激对睡眠剥夺大鼠焦虑情绪及学习记忆能力的影响
武霄铮张克英张俊平郭玲安广洲巫彤宁丁桂荣
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摘要:
目的 探讨超低频经颅磁刺激(TMS)对睡眠剥夺诱导的大鼠焦虑情绪和学习记忆损伤的 干预效果。方法 SD 大鼠18 只随机分为对照(con)组,睡眠剥夺假刺激(sham)组和睡眠剥夺经颅磁刺 激(TMS)组。采用改良多平台睡眠剥夺法建立大鼠睡眠剥夺模型,建模成功后,利用自制便携式TMS 仪 对大鼠头部施加TMS 刺激(连续15 个单刺激,刺激间隔30 s),每天刺激一次,连续刺激6 d。实验过程中 监测体重,采用旷场实验、高架十字迷宫和Morris水迷宫等行为学方法评价大鼠焦虑情绪和学习记忆能 力。结果 与con 组相比,sham组在睡眠剥夺5 d 后体重明显下降。旷场和高架十字迷宫实验显示,与 con 组相比,睡眠剥夺6 d 后sham组在中央区停留时间、开臂停留时间和进入次数百分比均明显降低。 Morris水迷宫显示,睡眠剥夺5 d 后,sham组到达平台潜伏期较con 组明显延长,且睡眠剥夺6 d 后的空 间探索实验中,sham组大鼠在目标象限停留的时间较con 组明显缩短。TMS 组与sham组各项指标差异 均无统计学意义。结论 超低频TMS 处理对睡眠剥夺诱导的动物焦虑情绪和学习记忆损伤未产生明显 的干预效果。
关键词:  睡眠剥夺  经颅磁刺激,超低频  焦虑情绪  学习记忆
DOI:10.3969/j.issn.1009-6574.2018.06.008
基金项目:军事医学创新工程(16CXZ020);第四军医大学军事医学提升计划(2016TSA-004)
Effects of ultralow-frequency TMS on anxiety and cognition in sleep deprivation rats
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Abstract:
Objective To investigate the effects of ultralow-frequency transcranial magnetic stimulation (TMS) on sleep deprivation-induced anxiety and cognitive capability deficit in rats. Methods A total of 18 SD rats were randomly divided into three groups: control group, sham group and TMS group. The sleep deprivation model was established by using improved multi-platform sleep deprivation method and the head of rat was stimulated by a homemade portable TMS instrument. TMS was performed once a day with 15 consecutive single stimulation( interval between two single stimulation was 30 s) for 6 consecutive days. Body weight was monitored during the experiment. Behavioral analysis such as open field test, elevated plus maze and Morris water maze were adopted to evaluate the anxiety and cognitive capability in rats. Results Compared with con group, the body weight of sham group significantly decreased after 5 days' sleep deprivation. The results of open field test and elevated plus maze test showed that compared with con group, the percentage time in central area, percentage time in open arms, and percentage entries into open arms significantly decreased in sham group after 5 days' sleep deprivation. Morris water maze test showed that compared with con group, the latency to platform significantly increased, meanwhile, the duration in target quadrant significantly decreased in sham group after 6 days' sleep deprivation. There was no significant difference in above all indexes was found between TMS group and sham group. Conclusions Under this experimental condition, the ultralow-frequency TMS intervention cannot improve sleep deprivation induced anxiety and cognitive capability deficit in rats.
Key words:  Sleep deprivation  Transcranial magnetic stimulation, ultralow-frequency  Anxiety  Cognitive capability

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