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Notch 3表达抑制后血管平滑肌细胞增殖能力的变化
刘楠刘莹丁玉
0
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摘要:
目的 利用已成功建立的Notch 3 基因表达下调的血管平滑肌细胞(VSMCs)模型,检测 在不同培养环境下VSMCs增殖能力的变化,进一步了解Notch 3 表达抑制后VSMCs的生存状态。方 法 利用氧化低密度脂蛋白(ox-LDL)和胰岛素样生长因子-1(IGF-1)干预正常VSMCs 及Notch 3 表达下 调的VSMCs,倒置显微镜下观察细胞生长情况,CCK-8 检测细胞生长曲线变化。结果 ox-LDL 对照干 预组的生长曲线明显高于ox-LDL 阳性干预组;接种第1~4 天ox-LDL 对照干预组、IGF-1 阳性干预组与 IGF-1 对照干预组的生长曲线略高于其他各组,第5、6 天后此趋势更加明显,而3 组间增殖程度相似。 结论 ox-LDL 与IGF 对于Notch 3 信号系统的作用并不一致。当Notch 3 受到抑制,ox-LDL的促增殖作 用则明显减弱;而IGF-1 对VSMCs 的促增殖作用依然保留。
关键词:  Notch 3  血管平滑肌细胞  增殖
DOI:10.3969/j.issn.1009-6574.2018.09.013
基金项目:
Changes in proliferation of vascular smooth muscle cells after inhibition of Notch 3
()
Abstract:
Objective To detect the change of proliferation and further find out the survival state of vascular smooth muscle cells( VSMCs) after knockdowned the expression of Notch 3 by RNA interference in different surroundings. Methods Ox-LDL and IGF-1 were applied to intervene normal VSMCs and Notch knockdowned VSMCs. Cell growth was observed under inverted microscope. Cell growth curve was detected by CCK-8. Results The growth curve of the ox-LDL control group was obviously higher than that of the ox-LDL positive group. During the first four days, the growth curve of ox-LDL control group, IGF-1 positive group and IGF-1 control group were higher than that of the other groups. This trend was more obvious in the fifth and sixth day. The proliferative rate was similar among the 3 groups. Conclusions For Notch 3 signal system, ox-LDL and IGF had different effects. When Notch 3 was inhibited, the proliferation of ox-LDL decreased. However, the proliferation promoting effect of IGF-1 on VSMCs remained.
Key words:  Notch 3  Vascular smooth muscle cell  Growth

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