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BDNF基因多态性与精神分裂症发病及临床症状的 相关性
王玉花胡强杨超李平赵阿勐崔光成闫凤武刘艺涵李树忠田野
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摘要:
目的 探讨BDNF基因rs2030324和rs11030101多态性与精神分裂症发病及临床症状的关 系。方法 于2017年1月—2018年1月由齐齐哈尔市精神卫生中心招募200例精神分裂症患者(病例组) 及200 例健康对照(对照组)纳入研究。每位研究对象抽取5 ml 静脉血提取基因组DNA,对rs2030324 和 rs11030101 两个位点上下游500 bp 的序列设计引物,进行PCR 扩增,比较rs2030324 和rs11030101 两个 位点在两组人群中的基因频率分布及基因型分布。对病例组采用阳性与阴性症状量表(PANSS)评估临 床症状,分析PANSS 评分与不同基因型的相关性。结果 病例组和对照组在rs2030324 和rs11030101 两个位点基因频率分布和基因型分布均具有统计学意义(P< 0.05)。该两个位点的A 等位基因,都是 精神分裂症的易感基因,OR 值=1.532、1.391;95%CI=1.154~2.034、1.027~1.883。rs11030101 位点AT 基因型的患者与PANSS 阴性症状评分相关,AT基因型的患者阴性症状评分最高。结论 BDNF 基因 rs2030324、rs11030101 位点多态性与汉族人群精神分裂症的发病具有相关性,该两个位点的等位基因 A 均可能是精神分裂症发病的易感等位基因。rs11030101 位点多态性可能影响精神分裂症的临床表现。
关键词:  精神分裂症  脑源性神经营养因子  单核苷酸多态性  症状
DOI:10.3969/j.issn.1009-6574.2019.01.011
基金项目:齐齐哈尔市科学技术局社会发展项目(SFGG-201448)
Correlation of BDNF gene polymorphism with pathogenesis and clinical symptoms of schizophrenia
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Abstract:
Objective To explore the correlation of the polymorhisms of brain-derived neurotrophic factor( BDNF) gene rs2030324 and rs11030101 with the pathogenesis and clinical symptoms of schizophrenia. Methods A total of 200 patients with schizophrenia from Qiqihar Mental Health Center( case group) and 200 healthy controls( control group) were recruited from January 2017 to January 2018. Five milliliters of venous blood was extracted from each study object to extract genomic DNA. Primers were designed for the upstream and downstream of rs2030324 and rs11030101. PCR amplification was performed to compare the gene frequency distribution and genotype distribution of two loci between the two groups. Clinical symptoms were evaluated by Positive and Negative Syndrome Scale( PANSS) in the case group, and the correlation between PANSS score and different genotypes was analyzed. Results The frequency distribution and genotype distribution of rs2030324 and rs11030101 had statistical significance( P< 0.05) between the two groups. The A alleles at the two sites were all susceptible to schizophrenia, with OR values of 1.532 and 1.391,95%CI( 1.154-2.034) and( 1.027-1.883), respectively. Patients with rs11030101 AT genotype were associated with PANSS negative symptom score, and patients with AT genotype had the highest negative symptom score. Conclusions The polymorphisms of BDNF gene rs2030324 and rs11030101 are correlated with the incidence of schizophrenia in the Han population, and the alleles A of both sites may be the susceptible alleles of the onset of schizophrenia. rs11030101 polymorphism may affect the clinical manifestations of schizophrenia.
Key words:  Schizophrenia  Brain-derived neurotrophic factor( BDNF)  Single nucleotide polymorphism  Symptoms

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