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酸枣仁提取物对尼古丁行为敏感化大鼠模型的疗效 及作用
焦宇王也夫吴桐王小龙崔光成赵容杰
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摘要:
目的 观察酸枣仁提取物对大鼠尼古丁行为敏感化的治疗作用,并探讨其神经化学作用 机制。方法 36 只成年Sprague-Dawley(SD)大鼠分为生理盐水组(A 组)、单次尼古丁组(B 组)、尼古丁行 为敏感化模型组(C组)、酸枣仁低剂量治疗组(D组)、酸枣仁高剂量治疗组(E组)和高剂量酸枣仁组(F组)。 C、D和E组皮下注射尼古丁[0.4 mg(/ kg·d)]7 d,A、B、F组注射等体积生理盐水7 d,后停药3 d。在停药期, D、E 和F组分别灌胃给酸枣仁提取物每天1 次(低剂量组:60 mg/kg,高剂量组:180 mg/kg),A、B 和C 组 灌胃等体积饮用水。第3 天灌胃后1 h,除A 和F组外,其余各组大鼠给予激发剂量的尼古丁(0.4 mg/kg), 后立即利用大鼠自发活动量检测系统检测60 min 内大鼠自发活动量,并采用超高效液相色谱法检测大 鼠纹状体内多巴胺含量。结果 与A 和B 组相比,C 组大鼠的自发活动量明显增加,而D 组和E 组剂量 依赖性地抑制多次尼古丁所致自发活动量敏感化增加;但高剂量酸枣仁治疗本身(F组)没有明显改变 大鼠自发活动量。超高效液相色谱法检测显示,C组大鼠纹状体多巴胺含量与B组大鼠相比有明显增高, 而D组和E 组中尼古丁敏感化引起的纹状体多巴胺含量显著降低。结论 酸枣仁可改善尼古丁大鼠行 为敏感化,其作用机制可能与降低大鼠脑内纹状体内多巴胺含量有关。
关键词:  尼古丁  纹状体  多巴胺  行为敏感化  酸枣仁
DOI:10.3969/j.issn.1009-6574.2019.03.016
基金项目:齐齐哈尔医学院院内科研基金(QY2016GJ-01)
Therapeutic effect of Jujube seeds extract on repeated nicotine-induced behavioral sensitization in rats
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Abstract:
Objectives To observe the therapeutic effect of Jujube seeds extract on the sensitisation of rat nicotine behaviour, and to explore its neurochemical mechanism. Methods Thirty-six adult Sprague- Dawley( SD) rats were divided into normal saline group( group A), single nicotine group( group B), nicotine behavioural sensitisation model group( group C), and jujube seed low-dose treatment group( group D), high dose treatment group( group E) and high dose of jujube group( group F). Groups C, D and E were injected subcutaneously with nicotine[ 0.4 mg(/ kg·d)] for 7 days. Groups A, B and F were injected with the same volume of normal saline for 7 days, and then discontinued for 3 days. During the withdrawal period, the D, E and F groups were administered with the jujube extract once a day respectively( low dose group: 60 mg/kg, high dose group: 180 mg/kg), and groups A, B and C were administered with equal volume of drinking water. On the third day, 1 hour after gastrointestinal administration, except for the A and F groups, the other groups of rats were given an activated dose of nicotine( 0.4 mg/kg). Immediately after using the rat spontaneous activity detection system to detect the spontaneous activity of rats within 60 min, then the ultra-high performance liquid chromatography( HPLC) was used to detect the dopamine content in rat striatum. Results Compared with group A and group B, the spontaneous activity of rats in group C increased significantly, while the sensitisation of
Key words:  Nicotine  Corpus striatum  Dopamine  Behavioral sensitization  Jujube seeds extract

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