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颈动脉低灌注大鼠模型海马区IL-33 和cleaved-caspase-3的表达
孟德龙贾柯娟李艳张彬
0
()
摘要:
目的 探讨IL-33 在颈动脉低灌注导致的认知障碍中的可能作用。方法 36 只成年雄 性SD 大鼠随机分为3 组:假手术(S)组,模型2 周(L2w)组,模型4 周(L4w)组。Morris水迷宫测试各组大 鼠学习记忆能力;免疫组化法检测海马区cleaved-caspase-3 表达水平;Western-blot 法检测海马区IL-33 表达水平。结果 水迷宫测试、cleaved-caspase-3 表达和IL-33 表达,3 组之间比较差异均有统计学意 义(F=64.201,P < 0.05;F=233.558,P < 0.05;F=51.498,P < 0.05)。与S 组(20.32±6.30)s 比较,L2w 组 (46.67±9.49)s逃逸潜伏期显著延长(t=-4.902,P< 0.05);L4w 组(81.51±14.67)s与L2w 组比较,逃逸潜伏 期显著延长(t=-6.397,P< 0.05)。与S 组(1.31±1.19)比较,L2w 组(6.56±1.32)海马区cleaved-caspase-3 表达显著增加(t=-6.328,P< 0.05);与L2w 组比较,L4w 组(18.78±5.83)海马区cleaved-caspase-3 表达显 著增加(t=-14.733,P< 0.05)。与S 组(0.26±0.02)比较,L2w 组(0.3±0.04)海马区IL-33 表达有增加趋势, 但结果无统计学意义(t=-1.530,P=0.147);与L2w 组比较,L4w 组(0.49±0.06)海马区IL-33 表达显著增加 (t=-7.924,P< 0.05)。结论 IL-33 可能在脑组织低灌注导致的认知障碍中起作用。
关键词:  血管性痴呆  颈动脉低灌注  白介素-33  cleaved-caspase-3
DOI:10.3969/j.issn.1009-6574.2019.04.018
基金项目:
Expression of IL-33 and cleaved-caspase-3 in the hippocampus of rats with carotid artery hypoperfusion
()
Abstract:
Objectives To investigate the possible role of interleukin-33( IL-33) in cognitive impairment caused by hypoperfusion of the carotid artery. Methods A total of 36 adult male Sprague-Dawley (SD) rats were randomly divided into 3 groups: sham operation( S) group, Lesion 2 weeks( L2w) group, and Lesion 4 weeks( L4w) group. The Morris water maze was used to test the learning and memory ability of each group. The expression of cleaved-caspase-3 in the hippocampus was tested by immunohistochemistry. The expression of IL-33 in the hippocampus was tested by Western-blot method. Results The differences in the water maze test performance and the expression of cleaved-caspase-3 and IL-33 were statistically significant between the three groups( F=64.201,P<0.05;F=233.558,P<0.05;F=51.498,P<0.05). Compared with the S group( 20.32±6.30)s, the escape latency of the L2w group( 46.67±9.49) s was significantly prolonged (t=-4.902,P < 0.05); compared with the L2w group, the escape latency of the L4w group( 81.51±14.67)s was significantly prolonged( t=-6.397,P< 0.05). Compared with the S group( 1.31±1.19), the expression of cleaved-caspase-3 in the hippocampus in the L2w group( 6.56±1.32) was significantly increased( t=-6.328, P<0.05); compared with the L2w group, the L4w group's( 18.78±5.83) expression of cleaved-caspase-3 in the hippocampus was significantly increased( t=-14.733,P<0.05). Compared with the S group( 0.26±0.02), the expression of IL-33 in hippocampus in the L2w group( 0.3±0.04) increased, but the results were not statistically significant( t=-1.530,P=0.147); compared with the L2w group, the L4w group's expression of IL-33 in the hippocampus( 0.49±0.06) was significantly increased( t=-7.924,P<0.05). Conclusions IL-33 may play a role in cognitive impairment caused by hypoperfusion of brain tissue.
Key words:  Vascular dementia  Carotid artery hypoperfusion  Interleukin-33  Cleavedcaspase- 3

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