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双相Ⅱ型障碍伴冲动攻击行为患者的全基因组DNA 甲基化修饰研究
芹那尔·波拉提江邹韶红赵宗峰
0
()
摘要:
目的 探讨双相Ⅱ型障碍伴冲动攻击行为患者的全基因组DNA 甲基化修饰情况。 方法 纳入5 例有冲动攻击行为的双相Ⅱ型障碍患者(攻击组)及5 例无冲动攻击行为的双相Ⅱ型障碍 患者(非攻击组),采用Illumina 公司的人类450K 甲基化芯片技术对10 例患者的全血进行甲基化水平的 分析。利用R 软件对原始数据进行预处理并筛选出样本分组间的差异甲基化位点采用GCBI 在线分 析软件对差异候选基因进行功能富集分析。结果 (1)攻击组与非攻击组样本比较甲基化水平有差异。 (2)攻击组与非攻击组样本差异甲基化位点在染色体上的分布有差异。(3)按基因注释分类和CpG 岛注 释分类筛选攻击组与非攻击组样本间的差异甲基化区域,差异甲基化区域在11个注释区域内均有分布。 (4)GO 分析结果显示,差异甲基化位点参与信号传递等功能。Pathway 分析中,主要参与多巴胺能神经 突触信号通路、趋化因子信号通路。结论 有无冲动攻击行为的双相Ⅱ型障碍患者的全基因组DNA 甲 基化水平及差异甲基化DNA 在染色体上的分布均有差异,差异甲基化位点主要参与信号传递等。
关键词:  双相情感障碍  冲动攻击行为  基因甲基化
DOI:10.3969/j.issn.1009-6574.2019.06.009
基金项目:
Study on the whole genomic DNA methylation modification of impulsive aggression in patientswith bipolar Ⅱ disorder
Qin Naer·Bo Latijiang, Zou Shaohong, Zhao Zongfeng
()
Abstract:
Objective To explore the whole genome DNA methylation modification of impulsive aggression in patients with bipolar Ⅱ disorder. Methods The Illumina human 450k methylation chip technology was used to analyze the level of methylation of the whole blood genomic DNA samples of 5 cases of bipolar Ⅱ disorder patients with impulsive behavior and 5 cases of bipolar Ⅱ disorder patients without impulsive behavior. The R software was used to preprocess the raw data and select the differentiated methylation loci between sample groups. Finally, the GCBI online analysis software was used to analyze the functional enrichment of different candidate genes. Results (1)There was a difference in the methylation levels between the aggression group and the non-aggression group.( 2)There was a difference in the distribution of methylation loci on the chromosome between the aggression group and the non-aggression group.( 3)The differentiated methylation loci were classified by gene annotation classification and CpG island annotation classification, and the differentiated methylation loci were distributed in 11 annotation areas.( 4)The result of GO analysis showed that the differentiated methylation loci participated in signal transmission and other functions. The pathway analysis showed that the affected function was mainly related with dopaminergic synaptic and chemokine signaling pathway. Conclusions In this study, the whole genomic DNA methylation levels and the distribution of methylated DNA on the chromosome in the aggression group were different from the non-aggression group.
Key words:  Bipolar disorder  Aggression behavior  Gene methylation

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