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医用三氧对抑郁症模型小鼠行为学的影响及相关机制探究
陶红波刘斌刘燕芹
0
()
摘要:
目的 探讨医用三氧对抑郁症模型小鼠行为学的影响及相关机制。方法 选取30 只健 康雄性ICR 小鼠,建立抑郁症小鼠模型,并随机分为Sham组(不作抑郁处理+生理盐水)、模型组(抑郁 处理+生理盐水)以及低、中、高浓度组(抑郁处理+腹腔注射60、80、100 μg/ml医用三氧),每组6只。通 过旷场实验、绝望行为实验检测小鼠抑郁行为,通过HE 染色观察小鼠海马部位病理变化,采用Western blot 检测小鼠海马组织中炎性因子细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、肿瘤坏死因子-α (TNF-α)水平。结果 抑郁症模型小鼠旷场行为实验结果显示,模型组小鼠的水平穿越格数、直立次 数以及修饰次数显著少于Sham组(P< 0.05),排便粒数显著多于Sham组(P< 0.05),且中、高浓度组的 水平穿越格数、直立次数、修饰次数及排便粒数显著优于模型组(P < 0.05),低浓度组的水平穿越格数、 直立次数、修饰次数及排便粒数与模型组比较,差异无统计学意义(P> 0.05)。绝望行为实验结果显 示,模型组小鼠实验后4 min 连续不动时间显著长于Sham组(P < 0.05),各浓度组小鼠实验后4 min连 续不动时间显著短于模型组(P < 0.05),其中高浓度组小鼠差异最大。体重实验结果显示,第0、7、14、 21 天,模型组小鼠的体重显著低于Sham组(P < 0.05);第21 天,高浓度组的体重显著高于模型组(P < 0.05),其余时间的体重与模型组比较差异无统计学意义(P> 0.05);第7、14、21 天,低、中浓度组的体重 与模型组比较差异无统计学意义(P > 0.05)。显微镜观察结果显示,模型组小鼠海马部位小胶质细胞 数量[(23.64±3.57)个]显著多于Sham 组[(18.11±2.92)个](P< 0.05),高浓度组小鼠海马部位小胶 质细胞数量[(17.27±2.69)个]显著少于模型组(P < 0.05),中、低浓度组小鼠海马部位小胶质细胞数量 [(22.58±3.84)个、(20.03±3.21)个]与模型组比较,差异无统计学意义(P > 0.05)。Western blot 结果显 示,模型组小鼠海马组织中炎性因子IL-1β、IL-6、TNF-α 水平显著高于Sham 组(P< 0.05),高浓度组小 鼠海马组织中炎性因子IL-1β、IL-6、TNF-α 水平显著低于模型组(P< 0.05);中、低浓度组小鼠海马组 织中炎性因子IL-1β、IL-6、TNF-α 水平与模型组比较,差异无统计学意义(P> 0.05)。结论 高浓度医 用三氧能够改善抑郁症模型小鼠抑郁样行为,其相关机制可能与下调脑组织中炎性因子表达有关。
关键词:  抑郁症  医用三氧  行为学  小鼠  炎症
DOI:10.3969/j.issn.1009-6574.2021.06.006
基金项目:湖北省武汉市卫生计生委科研项目(320.2199)
Effects of medical ozone on the behaviors of depression model mice and its related mechanisms
Tao Hongbo, Liu Bin, Liu Yanqin
()
Abstract:
Objective To explore the effects of medical ozone on behaviors of depression model mice and its related mechanisms. Methods A total of 30 healthy male ICR mice were selected to establish depression model, and randomly divided into Sham group( no depression treatment + normal saline), model group( depression treatment + normal saline), and low, medium, and high concentration groups( depression treatment + intraperitoneal injection of 60μg/ml, 80μg/ml and 100μg/ml medical ozone), with six mice in each group. Depressive behaviors of mice were detected by open field experiment and behavioral despair experiment. Pathological changes in the hippocampus of mice were observed by HE staining. The levels of inflammatory cytokines of interleukin-1β( IL-1β), interleukin-6( IL-6) and tumor necrosis factor-α( TNF-α) in hippocampus of mice were detected by Western blot. Results The open field behavior experiment results of depression model mice showed that the number of horizontal crossing lattice, the number of uprights, and the number of modifications in model group were significantly less than those in Sham group( P< 0.05), and the number of defecation grains was significantly more than that in Sham group( P<0.05), and the indexes in medium and high concentration groups were significantly better than those in model group( P<0.05), and there were no significant differences between low concentration group and model group( P > 0.05). The behavioral despair experiment results showed that the 4 min continuous immobility time of mice in model group after experiment was significantly longer than that in Sham group( P < 0.05), and the time in each concentration group was significantly shorter than that in model group( P<0.05). Among them, the mice in high concentration group had the largest difference. The results of body mass test showed that the body mass on the 0 d, the 7th d, the 14th d and the 21st d of mice in model group was significantly lower than that in Sham group( P<0.05). On the 21st d, the body mass in high concentration group was significantly higher than that in model group( P< 0.05), and there was no significant difference in the rest of the time compared with that in model group( P > 0.05). On the 7th d, the 14th d and the 21st d, there was no significant difference among low and medium concentration groups and model group( P>0.05). The results of microscope observation showed that the number of microglia in the hippocampus of model group was significantly more than that of Sham group[ (23.64±3.57) vs( 18.11±2.92)]( P < 0.05), and that of high concentration group( 17.27±2.69) was significantly less than that of model group( P<0.05), and there was no significant difference among low( 20.03±3.21) and medium (22.58±3.84) concentration groups and model group( P>0.05). Western blot results showed that the levels of inflammatory factors IL-1β, IL-6, and TNF-α of hippocampus of model group were significantly higher than those of Sham group( P < 0.05), and those of high concentration group were significantly lower than those of model group( P< 0.05), and there was no significant difference among low and medium concentration groups and model group( P > 0.05). Conclusions High-concentration medical ozone can improve depression-like behaviors in depression model mice, and its possible related mechanism is related to the down-regulation of expression of inflammatory levels factors in brain tissue.
Key words:  Medical ozone  Depression  Behaviors  Mice  Inflammation

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